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Objective:To compare the effectiveness between three methods for purifying the immunoglobulin of egg yolk(IgY) which are polyethylene glycol (PEG) method, chloroform extraction method and chloroform / PEG method, and to provide basis for obtaining the batch of IgY.Methods:The inactivated vaccine of Vibrio parahemolyticus (V. parahemolyticus) was prepared and the hens were immunized by multi-point intramuscular injection.The eggs were collected and the IgY was purified by PEG method, chloroform extraction method and chloroform/PEG method.The protein extraction rate, the IgY titer and the purity of the antibody which purified by different methods were detected.Furthermore, the operation process, cost and safety of the three methods were analyzed.Results:The protein contents of the extraction belonging three methods from high to low in turn were chloroform extraction method, chloroform/PEG method, and PEG method.There was no significant difference in the antibody titer between three methods, and the tiler of chloroform extraction method was slightly high.The purities of purified antibody from high to low in turn were PEG method, chloroform/PEG method and chloroform method.The PEG method had better security but relatively lower extraction efficiency and higher cost.The chloroform/PEG method had high extraction efficiency and good antibody purity.Conclusion:The PEG method is suitable for a small amount of extraction in the laboratory.The chloroform/PEG method is appropriate for extracting the high quality IgY in a batch as it has high extraction efficiency and good antibody purity.
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Abstract BACKGROUND: Large amounts of data have shown that Chinese herbal monomer has a neuroprotective effect, and can improve the quality of life in stroke patients with cerebral nervous system injury. Nuclear-factor-erythroid 2-related (Nrf2) factor has neuroprotective effect on hemorrhagic stroke and ischemic stroke, which is an important way to reverse the damage of nervous system through the natural non-toxic Chinese herbs or composition, but it is rarely reported systemicaly. OBJECTIVE:To summarize the neuroprotective effect of Chinese herbal monomersvia the Nrf2 signal pathway in stroke patients. METHODS:The first author retrieved the CNKI, VIP, Medline, and PubMed databases by computer. The keywords were “Nrf2, ARE, stroke, traditional Chinese medicine, neuroprotection” in Chinese and English,respectively. Articles concerning the neuroprotective role of Chinese herbal monomervia Nrf2 were analyzed and discussed. RESULTS AND CONCLUSION: Totaly 85 articles were retrieved. According to the inclusion and exclusion criteria, 46 articles were included in result analysis. The results show that a variety of monomers can exert neuroprotective effectsvia the Nrf2 pathway. Chinese herbal monomers include organic acids, phenols, saponins, terpenes, flavonoids, alkaloids, and other single or composite components. Traditional Chinese medicine has the clear neuroprotective effect after stroke, but it is lack of regularity, and it is stil need to expand the data and further research as the basis.
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BACKGROUND: In the evaluation on the effects of intervention on the patients with angina pectoris,whether the quality of life is improved or not is a very important factor while the symptoms and physical signs are benefited.OBJECTIVE: To probe into the intervention effects of sini tang,the classic formula,on angina pectoris of coronary heart disease and compare the effects of simple Chinese herbs,western medicine and integrated traditional Chinese and western medicine on quality of life.DESIGN:Prospective observation and control study in which the patients with angina pectoris of coronary heart disease were taken as the objects.SETTING: Department of Traditional Chinese Medicine of a Hospital Affiliated to a University.PARTICIPANIT : The 88 cases in total were the patients with angina pectoris of coronary heart disease in the clinic of Department of Traditional Chinese Medicine of First Hospital Affiliated to Sun Yat-sen University from February to August 2003. Inclusion criteria: those compiled with "Guide Principles of Pharmaceutical Clinical Study in Cardiac Vascular System" and "Standards on Differentiation of Coronary Heart Disease with Chinese Medicine". Exclusion criteria: those complicated with various severe diseases. All the patients were randomized into sini tang group,isosrbide dinitrate group and allied medication group of 29,30 and 29 cases successively.METHODS: In sini tang group,isosrbide dinitrate group and allied medication group,sini tang,isosrbide dinitrate and sini tang allied with isosrbide dinitrate were prescribed successively. At the beginning and the end of treatment, it was to observe the attack frequency,condition, severity and persistent duration of angina pectoris and to record electrocardiogram (ECG).The evaluation on quality of life was carried on before and two weeks after medication with the unified inventory and determination device.MAIN OUTCOME MEASURES: Comparisons of therapeutic effect on angina pectoris control,ECG and evaluation on life quality in every group.RESULTS: Both isosrbide dinitrate and sini tang relieved remarkably angina pectoris, improved ECG and quality of life in physical symptoms, health pleasure and depression level(P<0.01) . The result of sini tang on improving the quality of life in above-mentioned three aspects was better than that of isosrbide dinitrate(P<0.01) . But the allied medication improved clinical therapeutic effects and quality of life (health pleasure and depression level) best(P<0.01).CONCLUSION: Sini tang benefits the improvement of quality of life of patients with angina pectoris. But during clinical medical intervention,the advantages of integrated traditional Chinese and western medicine should be adopted.
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BACKGROUND:The therapeutic effect of traditional Chinese drug on myocardial ischemia and reperfusion has been increasingly attached importance to. The tongxinluo, a Chinese paten drug taking insect medicine as its major gradients, has been achieved satisfactory effect in anti-myocardial ischemia clinicallyOBJECTIVE: To investigate the effect of tongxinluo on myocardial apoptosis induced by ischemic reperfusion.DESIGN:A randomized controlled trialSETTING: Department of Traditional Chinese Medicine (TCM) of the First Affiliated Hospital, Sun Yat-sen UniversityMATERIALS:The experiment was conducted from November to December 2002 at the Molecular Medicine Research Center of Sun Yat-sen University.METHODS:Totally 72 Kunming mice were at random divided into 6groups with 12 in each group: 2 g/kg tongxinluo, 1.5 g/kg tongxinluo, 1.0g/kg tongxinluo group (The mice were administrated by gavage, once a day, on the day 5, one hour after administration, pituitrin and nitroglycerin were in combination used for building the myocardial ischemic reperfusion model), isosorbide dinitrade group (The dose was 3.9 g/kg, and the rest treatment was the same as the above), model group and blank control group(Normal saline was taken as substitute for the drug). At the end of trial the myocardia of the mice were taken for investigating the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of myocardia in mice with ischemic reperfusion, analyzing the myocardial apoptosis index, and determining the expression levels of apoptosis-relevant proteins bcl-2 and bax.MAIN OUTCOME MEASURES: [1] Comparison of SOD activity and MDA level in mice of each group. [2] Comparison of myocardial apoptosis and apoptosis relevant proteins.RESULTS:Totally 72 mice involved were good for the trial and no one died. [1] SOD activity and MDA level: Compared with the model and isosorbide dinitrade groups, SOD activity in 2 g/kg tongxinluo, 1.5 g/kg tongxinluo, 1.0 g/kg tongxinluo group was increased [( 1379.28±140.03 ),(1223.91 ±109.02), (1789.19 ±155.20), (2267.79 ±216.04), (2387.55±229.71 )nkat/L]; MDA level was decreased [(16.58±2.59), (20.40±2.66),(12.73±1.98), (9.65±2.03), (7.56±1.67)nmol/L], and the effect was increased as the dose was raised [2] myocardial apoptosis and apoptosis relevant proteins: Compared with the model and isosorbide dinitrade groups, the myocardial apoptosis index and Bax expression index in 2 g/kg tongxinluo,1.5 g/kg tongxinluo, 1.0 g/kg tongxinluo group were obviously decreased[(13.84±1.97)%, (11.56±1.43 )%, ( 334±0.96)%, (0.82±0.12 )%, (0.42±0.06)%;(17.44±6.18)%, (15.45±3.72)%, (10.85±2.73)%, (6.46±1.88)%, (5.57±1.49)% ]; Bcl-2 expression index was obviously increased [(6.22±0.50)%, (8.73 ±0.63)%, (11.38 ±1.38)%, (16.22 ±2.36)%, (19.45±2.92)%], and with a dose-effect relationship.CONCLUSION:Tangible capsule had the effects of. resisting oxygen free radical injury, anti-myocardial apoptosis and protecting ischemic reperfusion myocardial cells, and with a dose dependence.